Systems Integration

Harvester Integration

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  Robotic, In Vitro
  Cell Culture Systems

Integration Process

Sample Systems

    Systems Integration: Robotic In Vitro Systems

Overview

Process

Sample System

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Sample In Vitro System:
Automated Growth of Caco-2 Membranes in 6-Well SnapWell® Plates

1. Starting

Load new plates into incubator.

2. Coating

Remove plate from incubator, transfer to Hood 1 and remove lid

Move plate to coating station, release

Coat plate, inner wells with 0.2 ml collagen

Regrip plate and replace lid

Allow plate to dry in Hood 1 tower for 4-5 hours

3. Conditioning

Remove coated plate from Hood 1, transfer to Hood 2 and remove lid

Place plate under camera, view/locate inserts

Place plate under dispenser; dispense 2.0 ml SFM into outer wells

Replace plate lid, transfer plate to incubator; incubate for 2 hours

Remove plate from incubator, transfer to Hood 2 and remove lid

Place plate in dispenser, dispense 0.5 ml of SFM into inner wells

Replace plate lid, return plate to incubator; incubate for 1 hour

Remove plate from incubator, transfer to Hood 2 and remove lid

Place plate under camera, view/locate inserts

Place plate under aspirator, aspirate media from outer wells

Place plate under dispenser, dispense 2.0 ml MWS into outer wells

Replace plate lid, place in incubator for at least 30 minutes

After last plate is conditioned begin seeding

4. Seeding

Cell suspension is maintained @ 37°C, then shaken gently with 5% CO2 to maintain proper pH and keep cells in suspension.

Remove plate from incubator, transfer to Hood 2 and remove lid

Place plate under aspirator, aspirate from inner well

Replace plate lid, transfer plate to Hood 1 and remove lid

Place plate into seeding station, seed plate with 0.5 ml into inner well

Replace plate lid, return plate to incubator; incubate for 48 hours

Re-feed (see feeding below)

5. Feeding

Remove plate from incubator after 48-hour incubation

Regrip plate, bring into Hood 2 and remove lid

Place plate under camera to view/locate inserts

Place plate under aspirator, aspirate media from outer wells, inner wells

Move plate to dispenser; dispense 0.5 ml MWS into inner wells and 2.0 ml MWS into outer well

Replace plate lid, return plate to incubator and incubate for 48 hours

Re-feed after 48 hours for a total of 21 days

Programmed protocols guide the operator in setting up the various steps in the procedure but can be modified to suit application and preference. Different tests and plate types may require different protocols.

Throughout the procedure, management software is optimizing all movements and functions of workstation components.